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normal fetal lung fibroblast cell line  (ATCC)


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    ATCC normal fetal lung fibroblast cell line
    Domatinostat preferentially inhibits cell growth and induces cell death in glioma stem cells. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung <t>fibroblasts</t> were treated with the indicated concentrations of domatinostat for 1–4 days, and the number of viable cells was assessed by trypan blue dye exclusion. ( B ) Cells were treated as in ( A ), and the percentage of dead cells was evaluated using the trypan blue dye exclusion assay. * p < 0.05 vs. treatment without domatinostat (i.e., at 0 nM).
    Normal Fetal Lung Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal fetal lung fibroblast cell line/product/ATCC
    Average 99 stars, based on 962 article reviews
    normal fetal lung fibroblast cell line - by Bioz Stars, 2026-02
    99/100 stars

    Images

    1) Product Images from "HDAC Class I Inhibitor Domatinostat Induces Apoptosis Preferentially in Glioma Stem Cells Through p53-Dependent and -Independent Activation of BAX Expression"

    Article Title: HDAC Class I Inhibitor Domatinostat Induces Apoptosis Preferentially in Glioma Stem Cells Through p53-Dependent and -Independent Activation of BAX Expression

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms26167803

    Domatinostat preferentially inhibits cell growth and induces cell death in glioma stem cells. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung fibroblasts were treated with the indicated concentrations of domatinostat for 1–4 days, and the number of viable cells was assessed by trypan blue dye exclusion. ( B ) Cells were treated as in ( A ), and the percentage of dead cells was evaluated using the trypan blue dye exclusion assay. * p < 0.05 vs. treatment without domatinostat (i.e., at 0 nM).
    Figure Legend Snippet: Domatinostat preferentially inhibits cell growth and induces cell death in glioma stem cells. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung fibroblasts were treated with the indicated concentrations of domatinostat for 1–4 days, and the number of viable cells was assessed by trypan blue dye exclusion. ( B ) Cells were treated as in ( A ), and the percentage of dead cells was evaluated using the trypan blue dye exclusion assay. * p < 0.05 vs. treatment without domatinostat (i.e., at 0 nM).

    Techniques Used: Exclusion Assay

    Domatinostat induces apoptotic cell death. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung fibroblasts were treated without or with 500 nM domatinostat for 1–3 days, and were then subjected to Western blot analyses of the indicated proteins. The numbers below the images represent the means ( n = 2) of the relative band intensities after each band was quantified by densitometry and normalized to the GAPDH value. * p < 0.05 vs. cells before domatinostat treatment (i.e., cells at 0 days) for each of the glioma stem cell lines and their differentiated counterparts, except for the right most panel where comparisons were made with the adjacent left lane (i.e., IMR-90 treated with domatinostat for 3 days). ( B ) Cells were treated without or with 500 nM domatinostat in the absence or presence of 40 μM Z-VAD-fmk for 1 day, and were then subjected to Western blot analyses of the indicated proteins. * p < 0.05 vs. cells treated with domatinostat alone. ( C ) Cells were treated as in ( B ), and were then subjected to the propidium iodide (PI) incorporation assay to assess the percentage of dead cells. * p < 0.05. In ( A , B ), the expected migrating position for cleaved caspase-3 is indicated by an arrow.
    Figure Legend Snippet: Domatinostat induces apoptotic cell death. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung fibroblasts were treated without or with 500 nM domatinostat for 1–3 days, and were then subjected to Western blot analyses of the indicated proteins. The numbers below the images represent the means ( n = 2) of the relative band intensities after each band was quantified by densitometry and normalized to the GAPDH value. * p < 0.05 vs. cells before domatinostat treatment (i.e., cells at 0 days) for each of the glioma stem cell lines and their differentiated counterparts, except for the right most panel where comparisons were made with the adjacent left lane (i.e., IMR-90 treated with domatinostat for 3 days). ( B ) Cells were treated without or with 500 nM domatinostat in the absence or presence of 40 μM Z-VAD-fmk for 1 day, and were then subjected to Western blot analyses of the indicated proteins. * p < 0.05 vs. cells treated with domatinostat alone. ( C ) Cells were treated as in ( B ), and were then subjected to the propidium iodide (PI) incorporation assay to assess the percentage of dead cells. * p < 0.05. In ( A , B ), the expected migrating position for cleaved caspase-3 is indicated by an arrow.

    Techniques Used: Western Blot



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    Image Search Results


    Domatinostat preferentially inhibits cell growth and induces cell death in glioma stem cells. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung fibroblasts were treated with the indicated concentrations of domatinostat for 1–4 days, and the number of viable cells was assessed by trypan blue dye exclusion. ( B ) Cells were treated as in ( A ), and the percentage of dead cells was evaluated using the trypan blue dye exclusion assay. * p < 0.05 vs. treatment without domatinostat (i.e., at 0 nM).

    Journal: International Journal of Molecular Sciences

    Article Title: HDAC Class I Inhibitor Domatinostat Induces Apoptosis Preferentially in Glioma Stem Cells Through p53-Dependent and -Independent Activation of BAX Expression

    doi: 10.3390/ijms26167803

    Figure Lengend Snippet: Domatinostat preferentially inhibits cell growth and induces cell death in glioma stem cells. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung fibroblasts were treated with the indicated concentrations of domatinostat for 1–4 days, and the number of viable cells was assessed by trypan blue dye exclusion. ( B ) Cells were treated as in ( A ), and the percentage of dead cells was evaluated using the trypan blue dye exclusion assay. * p < 0.05 vs. treatment without domatinostat (i.e., at 0 nM).

    Article Snippet: IMR-90, a human normal fetal lung fibroblast cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA) and maintained in DMEM supplemented with 10% FBS.

    Techniques: Exclusion Assay

    Domatinostat induces apoptotic cell death. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung fibroblasts were treated without or with 500 nM domatinostat for 1–3 days, and were then subjected to Western blot analyses of the indicated proteins. The numbers below the images represent the means ( n = 2) of the relative band intensities after each band was quantified by densitometry and normalized to the GAPDH value. * p < 0.05 vs. cells before domatinostat treatment (i.e., cells at 0 days) for each of the glioma stem cell lines and their differentiated counterparts, except for the right most panel where comparisons were made with the adjacent left lane (i.e., IMR-90 treated with domatinostat for 3 days). ( B ) Cells were treated without or with 500 nM domatinostat in the absence or presence of 40 μM Z-VAD-fmk for 1 day, and were then subjected to Western blot analyses of the indicated proteins. * p < 0.05 vs. cells treated with domatinostat alone. ( C ) Cells were treated as in ( B ), and were then subjected to the propidium iodide (PI) incorporation assay to assess the percentage of dead cells. * p < 0.05. In ( A , B ), the expected migrating position for cleaved caspase-3 is indicated by an arrow.

    Journal: International Journal of Molecular Sciences

    Article Title: HDAC Class I Inhibitor Domatinostat Induces Apoptosis Preferentially in Glioma Stem Cells Through p53-Dependent and -Independent Activation of BAX Expression

    doi: 10.3390/ijms26167803

    Figure Lengend Snippet: Domatinostat induces apoptotic cell death. ( A ) GS-Y01, GS-Y03, TGS01, their differentiated counterparts (dGS-Y01, dGS-Y03, and dTGS01), and IMR-90 human lung fibroblasts were treated without or with 500 nM domatinostat for 1–3 days, and were then subjected to Western blot analyses of the indicated proteins. The numbers below the images represent the means ( n = 2) of the relative band intensities after each band was quantified by densitometry and normalized to the GAPDH value. * p < 0.05 vs. cells before domatinostat treatment (i.e., cells at 0 days) for each of the glioma stem cell lines and their differentiated counterparts, except for the right most panel where comparisons were made with the adjacent left lane (i.e., IMR-90 treated with domatinostat for 3 days). ( B ) Cells were treated without or with 500 nM domatinostat in the absence or presence of 40 μM Z-VAD-fmk for 1 day, and were then subjected to Western blot analyses of the indicated proteins. * p < 0.05 vs. cells treated with domatinostat alone. ( C ) Cells were treated as in ( B ), and were then subjected to the propidium iodide (PI) incorporation assay to assess the percentage of dead cells. * p < 0.05. In ( A , B ), the expected migrating position for cleaved caspase-3 is indicated by an arrow.

    Article Snippet: IMR-90, a human normal fetal lung fibroblast cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA) and maintained in DMEM supplemented with 10% FBS.

    Techniques: Western Blot